Analysis of bacterial proteomes - INRA de Clermont-Ferrand - Theix, UR454 Microbiologie
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The proteomic approaches undertaken by the team BaDiAl concern the analysis of subproteomes (intracellular proteins, membrane, parietal, surface-exposed, exoproteins) of (i) Listeria monocytogenes, a foodborne pathogen bacterium, (ii) Staphylococcus xylosus, a bacterium used as a starter in meat and dairy products and (iii) Escherichia coli, a commensal bacterium of the mammal intestinal tract, whose some strains can cause serious illnesses.

The research topics using proteomic approaches concern the mechanisms of adhesion and biofilm formation, the characterization of mutant strains, particularly those affected in protein secretion pathways, the adaptive response to environmental stresses or technological processes, characterization / comparison of extended spectrum β-lactamase (ESBL) producing-E. coli.

 

 

The methodologies used depend on the objectives of research, they use different techniques for protein or peptide extraction, for protein separation in-gel (SDS-PAGE, 2-DE) and / or off-gel (1-D or 2-D LC ) and for protein identification by MALDI-TOF/TOF (Autoflex speed, Bruker) or ESI-MS/MS (LTQ Velos, Thermo Scientific) mass spectrometry coupled (or not) to liquid chromatography. Comparative analysis of 2-DE gels is performed with Progenesis SameSpot software (Nonlinear Dynamics).

The Research Unit 454 works with the proteomic component of the Platform of Metabolism Exploration (PFEMcp) at INRA in Clermont-Ferrand/Theix (http://www5.clermont.inra.fr/plateforme_exploration_metabolisme) for mass spectrometry, analysis and comparisons of data (Progenesis LC-MS, Nonlinear Dynamics) and the databases query (Mascot, PEAKS, Sequest).

 
Contacts :  Michel Hébraud - Ingrid Chafsey

Last update: 17 Juillet, 2012

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